4 Questions 4 Answers 0 Followers
Questions related from Carrie Oh
After synthesising cDNA today I noticed some residue on the seal and on the walls. Most of the residue on the top, on the seal, came down upon centrifugation... However om The walls the tiny...
10 April 2018 9,461 3 View
Hello! I have averaged the duplicates of my raw Ct values and have used the delta delta Ct method to get a fold-change for each of the data within each subgroup. Each subgroup has around 4-6 lots...
07 April 2018 8,499 3 View
Hello (again), I will write this in as simple terms as possible. I have two diet types, let's call one Normal, the other HF. I have WT and TG mice participating in each diet group. Therefore 4...
30 March 2018 4,000 4 View
Hello! I am running qPCR with SYBR green protocol, with an annealing temperature of 60°C for time:01:00 for all my primers. The results have been fine for primers with 20 nts and with Tm under 60....
28 March 2018 9,195 5 View
