Carolina Melero-Jerez

6 Questions 5 Answers 0 Followers

Questions related from Carolina Melero-Jerez

How can I deplete IFNß specific T cell response?

I would like to separate the in vivo IFN-ß effects observed in the EAE model attending to different subsets. For that purpose, I would need to specifically deplete the intracellular pathway for...

11 July 2017 5,409 5 View

How could we eliminate astrocytes from a mixed glial cell culture?

We are trying to obtain a pure oligodendrocyte culture from p0 mice. After one week of mixed glial culture in ornitine-coated flasks, we purified the oligodendrocyte precursor cells (OPCs) by...

22 March 2016 4,573 8 View

Which medium is the best for ATCC A2B5 hybridome culture?

Hi everyone! I am trying to culture cells from ATCC (ref: CRL-1520-ATCC) in order to obtain the A2B5 antibody. As the company recommends, I use a DMEM formulation from Sigma-Aldrich (D-7777) with...

25 January 2016 8,158 3 View

Any reason for the absence of policlonal activation of CD3+ T-cells in culture?

Hi everybody, I've been growing T cells from a flow cytometry CD3-sorting for a long time, obtained from C57BL6 mice spleens. At least three hours before seeding, the p96-well plate is coated...

09 June 2015 9,844 6 View

Any good Foxp3 antibody for IHC in spinal cord?

Does anybody know a good antibody for the detection of T regs in spinal cord? The IHC would combine CD4/CD8, CD25 and Foxp3. Thank you in advance.

18 March 2015 9,107 3 View

Does anybody have a protocol for freezing MDSCs?

I would like to freeze MDSCs obtained after a FACS sorting. Has anybody a specific protocol for doing that? Or should I use just fetal bovin serum with DMSO? Thank you in advance.

14 November 2014 9,588 3 View