Carolina Melero-Jerez

7 Questions 5 Answers 0 Followers

Questions related from Carolina Melero-Jerez

How can we analyse the OCT data of demyelination?

We are analysing the demyelination and remyelination of the optic nerve using OCT. We wondering how to do the proper read out. We are not sure if the higher the hole is the bigger the...

18 June 2018 7,539 4 View

How can I deplete IFNß specific T cell response?

I would like to separate the in vivo IFN-ß effects observed in the EAE model attending to different subsets. For that purpose, I would need to specifically deplete the intracellular pathway for...

11 July 2017 5,431 5 View

How could we eliminate astrocytes from a mixed glial cell culture?

We are trying to obtain a pure oligodendrocyte culture from p0 mice. After one week of mixed glial culture in ornitine-coated flasks, we purified the oligodendrocyte precursor cells (OPCs) by...

22 March 2016 4,598 8 View

Which medium is the best for ATCC A2B5 hybridome culture?

Hi everyone! I am trying to culture cells from ATCC (ref: CRL-1520-ATCC) in order to obtain the A2B5 antibody. As the company recommends, I use a DMEM formulation from Sigma-Aldrich (D-7777) with...

25 January 2016 8,186 3 View

Any reason for the absence of policlonal activation of CD3+ T-cells in culture?

Hi everybody, I've been growing T cells from a flow cytometry CD3-sorting for a long time, obtained from C57BL6 mice spleens. At least three hours before seeding, the p96-well plate is coated...

09 June 2015 9,865 6 View

Any good Foxp3 antibody for IHC in spinal cord?

Does anybody know a good antibody for the detection of T regs in spinal cord? The IHC would combine CD4/CD8, CD25 and Foxp3. Thank you in advance.

18 March 2015 9,120 3 View

Does anybody have a protocol for freezing MDSCs?

I would like to freeze MDSCs obtained after a FACS sorting. Has anybody a specific protocol for doing that? Or should I use just fetal bovin serum with DMSO? Thank you in advance.

14 November 2014 9,602 3 View