How to illustrate FE Panel Regression with LSDV in a research framework?

First time to use FE Panel Data Regression using LSDV, and not familiar with how to illustrate it in a research framework. We have regional CSR score as IV and Social Development ( four areas:...

14 January 2021 580 3 View

Context in a Quantitative Context Analysis?

Hello, I am writing my bachelor thesis right now and am conducting a quantitative context analysis looking at how terrorism is portrayed in newspapers, but certain words could be used to describe...

04 May 2020 8,762 3 View

Species that contains the grass allergen Phl p5?

Does anyone know in which species the grass allergen Phl p5 occurs? It was originally isolated from the grass species Phleum pratense so it must at least occur in that species. It stand to reason...

15 March 2020 8,703 4 View

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24 February 2020 6,469 1 View

Does anyone know a freeware tool that can align exon and intron sequences to a template DNA sequence?

I am seaqrching for a tool that can align exon and intron sequences to a template DNA sequence without disrupting any of the short sequences. I remember that DNAStar was able to do this. Does...

12 December 2019 8,902 3 View

Direction and Wind speed using 3D Ultrasonic Anemometers?

What is the most accurate way of calculating wind direction (2D NESW), wind speed and vertical wind movement using 3D Ultrasonic Anemometer data in the form of uX-uY-uZ m/s (or U-V-W vector) data?...

22 October 2019 8,475 4 View

What is the link between a country's export performance and its national innovation international ranking and or reputation?

What is the link between a country's export performance and its national innovation international ranking and or reputation?

20 March 2019 8,010 4 View

Where can I find the following Italian agricultural text?

Hi, I am looking for an Italian agricultural text which seem to be difficult to find, can anyone provide me with a .pdf or the location of where I can acquire the text? Barbieri R., Botarelli L.,...

29 January 2019 3,317 2 View

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Hi there, I need a bit help in statistics. I developed an analytical method, which relies on calibration curves. For calibration, the measured signal value of a calibrator is plotted against...

03 November 2018 7,402 6 View

Can I Store Cells in -80 for later protein sampling?

Hello, I wonder is it possible to store the cells (Plate) at -80 degrees Celsius for later protein sampling (western blot)? I want your opinions

01 November 2018 2,819 6 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Why is my GOI not expressing after lentiviral transduction despite RNA increase and protein is detected in transfection?

Hi, I'm trying to create a stable overexpression line with EF1a-FlagGOI-IRES-GFP using lentivirus packaged with pxPAX2 and pM2G. After transduction of 293T with 8ug/ml polybrene, I was able to...

29 January 2021 1,878 4 View

Can I RNase A treatment my extracted plamid?

I have extracted the plasmid with the Qiagen maxi Endofree kit. the result of gel electrophoresis for this sample showed a band of degraded RNAs. Now, I want to remove RNAs from the eluted plasmid...

25 January 2021 2,958 3 View

Can I check GFP transfection in 293T cells by flow cytometry?

I would like to troubleshoot the transfection stage of my lentiviral transduction protocol. In short, I have 4 lentiviral plasmids, one of which encodes GFP. I tried transfecting the HEK293T...

24 January 2021 2,341 3 View

How do you improve retroviral expression from PG13 cells?

I have been trying to express a gene (GFP) in a retroviral system by transfecting human Phoenix-ECO cells, and transducing murine PG13 cells with the supernatant. I then sort GFP-positive PG13...

18 January 2021 476 1 View

What is the best way of moscaicing overlapping images?

I have several images with overlaping areas. I try to mosaic them using rasterio.merge library. But rasterio.merge funtion replaces those overlapping pixels with "nan" values. I then tried both...

29 December 2020 9,849 1 View