Bumjun Kim

9 Questions 19 Answers 0 Followers

Questions related from Bumjun Kim

How to educate myself to omics? Any advice for self-learners?

Hi everybody, I am a bench-scientist, and have realized that incorporating omics into my research is very important to do more systemic analysis on the topic. Is there any good way to educate...

05 May 2019 2,004 5 View

Quantification method for IHC or TUNEL staining for primary tumor section?

Hi, I have a question for unbiased quantification method for tumor section staining. I am currently doing TUNEL assay on primary breast tumor (Triple negative breast cancers) sections from...

04 April 2019 6,831 2 View

Designing an experiment for ROS Mesaurement (Expert Help needed)?

Hi, I am planning to measure ROS levels in cancer cells treated with DNA intercalating drug(Dox) and enzyme inhibitor. I will have four groups; 1. No treatment(control), 2. Dox, 3. inhibitor, 4....

10 October 2018 9,027 14 View

Reviewing process for western blot Stain-free gel to compare a protein of interest in Cytoplasm vs Nucleus?

Hi I was wondering if anybody have used stain free gel (Biorad) to compare the expression of a protein of interest in Cytoplasm and Nucleus? If I were to submit a paper including this stain free...

09 September 2018 7,654 5 View

How to decide whether you want to run western blot or not from qPCR data? If the expression level of RNA is too low, any alternative?

Hi, I have problem detecting specific proteins using Western Blot. I am pretty sure that it is not the problem of my technique or antibody since I can still see loading proteins and ladders on...

08 August 2018 6,280 5 View

Diffused band on Western Blot gel?

Hi, I ran western blot with two proteins with increasing concentration from 5 ug to 25 ug ; b-actin(control, 42kDA, lane 2-5), and Foxm1(protein of interest, 100kDA, lane 6-10). They were from...

08 August 2018 3,074 3 View

How can I modify sulfonic acid group? Any viable chemistry?

Hi, I am trying to modify sulfonic acid group to conjugate with carboxylic group or amine group in protein. I have hard time finding chemistry to modify sulfonic acid for the conjugation. Can...

04 April 2018 5,189 5 View

Techniques to immobilize cell nucleus?

Hi, Has any one try to immobilize cell nucleus on dishes or slides? If you did, how did you do it? I am assuming that I might be able to do it with coating dishes with material having opposite...

04 April 2018 5,342 4 View

What is the process of ion complex formation of molecules in organic solvent?

Hi, I have a question in forming ionic complex. I see some people making ionic complex of partially ionizable drug to increase lipophilicty and incorporate it into the lipid matrix. However, a...

01 January 2018 7,993 4 View