Bumjun Kim

9 Questions 16 Answers 0 Followers

Questions related from Bumjun Kim

Immunofluorescence staining of macrophages in mouse intestines?

Hi I am interested in IF staining macrophages in frozen colon sections. I chose F4/80 as it is a pan-macrophage marker. However, I have hard time staining macrophages in mouse colons. Typically,...

27 February 2022 5,922 2 View

Need advice from IHC experts on my staining technique please?

Hi guys. I keep getting staining on void area of tissue (breast tumor tissues). As you can see both in my negative control(without primary antibody) and positive control, there are stretch...

17 May 2019 8,461 5 View

How to educate myself to omics? Any advice for self-learners?

Hi everybody, I am a bench-scientist, and have realized that incorporating omics into my research is very important to do more systemic analysis on the topic. Is there any good way to educate...

12 May 2019 2,913 4 View

Isotype control for polyclonal antibody when doing IHC?

Hi I have a question in setting up a control for background staining when doing IHC. I have a polyclonal antibody to stain tissue using IHC. I was going to use isotype control. However, it seems...

09 January 2019 2,971 3 View

Designing an experiment for ROS Mesaurement (Expert Help needed)?

Hi, I am planning to measure ROS levels in cancer cells treated with DNA intercalating drug(Dox) and enzyme inhibitor. I will have four groups; 1. No treatment(control), 2. Dox, 3. inhibitor, 4....

19 October 2018 3,641 13 View

How to decide whether you want to run western blot or not from qPCR data? If the expression level of RNA is too low, any alternative?

Hi, I have problem detecting specific proteins using Western Blot. I am pretty sure that it is not the problem of my technique or antibody since I can still see loading proteins and ladders on...

13 August 2018 8,637 3 View

Techniques to immobilize cell nucleus?

Hi, Has any one try to immobilize cell nucleus on dishes or slides? If you did, how did you do it? I am assuming that I might be able to do it with coating dishes with material having opposite...

29 April 2018 139 3 View

How do you know that which one will be reduced when you have two different reducing agent?

Hi, I have a question in redox process. It might be basic chemistry question but I cannot figure out. Can you help me understand the concept and theory behind it please? I was reading an article...

27 July 2017 1,847 0 View

What is the role of protonation/deprotonation in dissolving hydrophilic molecules in water? How important it is?

Hi, I have recently got a question on this topic. Decitabine (anticancer drug) has no charge throughout the entire pH region since high pKa value of hydroxyl group(~14) and extremely low pKa value...

12 May 2017 9,527 6 View