Bu Gayun

4 Questions 2 Answers 0 Followers

Questions related from Bu Gayun

Are these Cyanine5 maleimide same?

Hello, I'm currently conducting protein labeling using Cy5 maleimide from Cytiva (PA25031). So far, I have been using the Cy5 maleimide mono-reactive dye from Cytiva, but I recently discovered...

02 August 2023 9,069 2 View

SDS-PAGE air bubble between gel and glass plate?

Hello, I'm currently having an issue with making an SDS-PAGE gel. After polymerization, big air bubbles form between the gel and glass plate. Although it doesn't appear to have a significant...

05 July 2023 3,226 0 View

Freezing E.coli pellet before lysis to get higher expression protein?

Hi, I wonder why E.coli cell pellet freezing is recommended before the lysis during protein expression and purification process. Most of the commercial protein purification protocols recommend...

16 January 2023 4,644 6 View

Is WGA staining remained after subculture?

Hi. I've been used Di-dye for membrane staining, and now I am considering WGA(Wheat Germ Agglutinin)-AF conjugate looking forward to better staining. Because I want to mix cells of 2 color, I want...

29 July 2021 6,273 1 View