Brenda Gonzalez

5 Questions 9 Answers 0 Followers

Questions related from Brenda Gonzalez

How is variation in Seahorse XFe24 respirometry controlled?

I have transduce HEK293T cell lines and am attempting to characterize some cellular and mitochondrial phenotypes after KD of my gene of interest. Seahorse is one of my main proposed assays but I...

07 July 2018 4,282 1 View

What are the best transfectable cell lines to use for mitochondrial and senescence studies?

Does anyone know of an easily transfected cell line that is rich in mitochondria (amendable to mitochondrial study) and not immortal? I want to generate cell lines expressing specific genetic...

01 January 2017 9,115 4 View

What is the typical protocol for dissolving NMN and what dosage is typically used to administer NMN in human cell cultures?

I want to treat human mitochondrial disease cells with nicotinamide mononucleotide. What should I use to reconstitute drug, water or PBS? And does anyone know of any studies where they used NMN in...

11 November 2016 2,038 7 View

How do I grow primary B-cells in culture with mitochondrial assay end-points?

Hi RG community, I am fairly new to cell culture to begin with but have been successfully working with primary human fibroblasts, but now I want to do experiments using primary human B-cells and...

10 October 2015 3,220 0 View

What is the protocol for activation of NFkB via TNFalpha treatment in HepG2 cells?

I have read that many researchers use 20 ng/ml for 15-30 minutes in other cancer cell lines, is this suitable for hepatic carcinoma?

01 January 2015 2,975 4 View