Bishnu Neupane

5 Questions 9 Answers 0 Followers

Questions related from Bishnu Neupane

How is HPLC method qualification perform?

I am working in validation and qualification of new HPLC method. Your insights and feedback are greatly appreciated.

03 December 2023 8,491 4 View

What are the possible combinations of different solvent system for reverse phase HPLC?

I am trying to purify a small peptide using reverse phase HPLC. The peptide is soluble in isopropanol and insoluble in water. A solution of peptide in isopropanol produces precipitates on addition...

11 January 2022 2,161 5 View

Could you please provide a guide for a reverse phase HPLC system to purify the amide product from the coupling of amino-acid and primary amine?

I am preparing amides derivatives from coupling of amino acids and primary amines. For purification of products, I am struggling to find suitable solvent system. I tried with gradient mixture of...

28 November 2021 336 2 View

Why small molecules works good for neurotrophic agents?

The synthesis of small molecules as neurotrophic agents are gaining momentum for the treatment of neuro degenerative diseases. I want to explore the scope of such small molecules. I would...

21 April 2021 5,486 3 View

Why very low concentration of enolate is required for cyclization?

The question is related to the organic synthesis

03 April 2021 2,003 7 View