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Questions related from Binbin Cui
Dear all, I have been doing a qPCR recently. In order to eliminate the false positive result, I add some PEG. The effect is good. But i cannot found the mechanism of PEG in PCR. Can anyone...
01 January 2020 5,077 3 View
Dear all, I am learning to design the qPCR assays recently. But I just don't know why my negative controls always give positive signal( I have changed my design twice). I checked the formation of...
10 October 2019 7,152 3 View
Dear all, I am designing a pair of primers for PCR, but my target region has very severe secondary structure(eg. hairpin with 15-bp stem). Can anyone please tell me whether the secondary...
09 September 2019 6,891 6 View
Dear Sir/ Madam, I am designing a primer for microRNA reverse transcription. But some microRNAs like hsp-mir-21-5p has a strong secondary structure. It forms a stable hairpin. Can anyone please...
09 September 2019 6,772 3 View
Dear all, I am amplifying a target sequence of 65 bases, which is a cDNA sequence now. For some reasons, my PCR primer can only bind to the target sequence with 11 bases in the first PCR cycle....
10 October 2018 9,549 8 View
Dear all, I am detecting mRNAs with hairpin molecular beacon. The 5' end of the hairpin oligo is modified with BHQ1 and the 3' end FAM. After I dissolved the hairpin in NF water, I dispensed one...
08 August 2018 499 5 View
The non-ionic detergent can be used as additives in the PCR to reduce the secondary structure, which might be helpful for the low yield PCR. But it may also increase non-specific amplification....
04 April 2018 5,001 2 View
I am making hydrogel beads with microfluidic droplet. The aqueous phase is a mixture of acrylamide, AA/bis-acrylamide, APS, and water. The oil phase is fluoroOil and fluoroSufactant with 0.4%...
12 December 2017 4,464 3 View
I am planning to treat the mRNA with sodium metaperiodate, but I am not sure if the chemical will degrade my mRNA. Can anyone give me some hints? Much of gratitude!
06 June 2017 7,713 2 View
