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An enzyme protein overexpressed in bacteria needs to be purified to show its activity. Why doesn't the cell lysate show activity when added to the substrate?
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In the past few years, the activity of the metalloproteinase expressed by bacteria was very low, but recently several expressions have suddenly shown an increase in activity. The used Plasmid...
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Other than hydrogen peroxide, which oxidants can oxidize ferrous ions into Fe(III)? How do they rank in terms of oxidation capacity? Are there any organic or endogenous oxidants present in the cell?
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From the uv-vis spectra, it can be seen that metalloproteins combine with small molecules to form complexes. But it's not clear what the complex is. How to identify metal-containing proteins and...
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What are the differences in the reducibility and application of common reducing agents such as sodium dithionite, DTT, GSH, ascorbic acid, etc.? How to reduce the metal center of the protein from...
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As a classical biocatalyst, what is the normal range of the catalytic constant (Kcat) value of the typical enzymes? What does it mean if it's less than 1/s?
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Cytochrome P450 (CYP) enzymes are a well-known family of drug-metabolizing enzymes to catalyze mono-oxygenation reactions of an enormous number and variety of substrates. It has been previously...
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According to 2002/657/EC, minimum required performance limit (MRPL) means minimum content of an analyte in a sample, which at least hasto be detected and confirmed. It is intended to harmonise the...
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