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An enzyme protein overexpressed in bacteria needs to be purified to show its activity. Why doesn't the cell lysate show activity when added to the substrate?
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In the past few years, the activity of the metalloproteinase expressed by bacteria was very low, but recently several expressions have suddenly shown an increase in activity. The used Plasmid...
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From the uv-vis spectra, it can be seen that metalloproteins combine with small molecules to form complexes. But it's not clear what the complex is. How to identify metal-containing proteins and...
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As a classical biocatalyst, what is the normal range of the catalytic constant (Kcat) value of the typical enzymes? What does it mean if it's less than 1/s?
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According to 2002/657/EC, minimum required performance limit (MRPL) means minimum content of an analyte in a sample, which at least hasto be detected and confirmed. It is intended to harmonise the...
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