Bhawna Gauri

3 Questions 5 Answers 0 Followers

Questions related from Bhawna Gauri

Q can anybody advice me on a formation of enhanced conjugation efficiency for amide bond between mPEG-COOH and TAT-NH2 peptide?

I am trying to couple my mPEG-COOH polymer (Mw 2000) with TAT peptide (Mw 1339) using selective carbodiimide reaction. I ran different trials using EDC + NHS with different solvents such as DMSO...

01 January 2016 2,263 3 View

Can anyone tell me how to calculate thiols group concentration in a polymer (Chitosan) ?

we have determined absorbace by using Ellman's Reagent I have used following Procedure: 1. 0.5 mg of conjugate hydrated in 250 uL of milli pore water (i.e., 2mg/mL concentration). 2. ...

06 June 2015 10,003 8 View

How can I remove excess of methyl bromoacetate from a reaction mixture?

If I am reacting mPEG with Sodium Hydride to form Alkooxide ions and then reacting with methyl bromoacetate to form ether products. It is a basic willamson ether synthesis. I tried column but it...

07 July 2014 1,316 6 View