Bhavani Gopalakrishnan

7 Questions 5 Answers 0 Followers

Questions related from Bhavani Gopalakrishnan

HMC3 cells and expression of IL-6, TNF-a, TGF-beta by ELISA?

Hi All I am PhD student working with HMC3 cells. This paper The human microglial HMC3 cell line: where do we stand? A systematic literature review is useful. But I would like to know the...

22 April 2019 3,313 1 View

What is good negative control cell for S100 marker?

I tried even the primary rat fibroblast but it was positive for S100. Most of the cell lines were positive too. So, I would like to know what is the good negative control for S100...

31 January 2018 9,764 3 View

HMC3 cell line and M2 phenotype

I am planning to work on this cell line and explore the phenotype with our drug of interest. I would like to know if we can check for M2 phenotype. Moreover, does Il6 expression of this cell...

12 May 2017 7,279 0 View

Can isopropyl alcohol be categorized as organic solvent?

I am trying to clean a hamilton syringe and I should not be organic solvents. So, can isopropyl alcohol (70%) be still used.

06 March 2017 7,872 5 View

Sterotaxic injections in spinal cord of transgenic mice?

Hi, Can someone tell me of hoe to perform sterotaxic injections in T10-T12 white matter of spinal cord of mice and does anyone tell me where can I find sterotaxic atlas for spinal cord.

06 March 2017 1,532 4 View

I wish you sterotaxic injections of transgenic mice 4 months old in T12 segment of white matter of spinal cord. Has anyone done injections?

I would like to know the detailed procedure as well

04 March 2017 2,117 2 View

HAs anyone tried detection of IL-6 using LPS and ATP (priming) on monocytes/macrophages cells?

I am working on HMC3 cells and I see there is mixed responses on using LPS and ATP for IL-6 detection. I understand we use this LPS +ATP for release of IL-1β and IL-18. But I do know that IL-1β...

01 January 1970 9,591 3 View