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Questions related from Benjamin Samuel Goldschmidt
I am doing research on evanescent fields and I'm curious as to whether or not a decaying exponential field can have a complex shape if it passes through nanolithography scale structures. (Examples...
24 June 2017 7,012 4 View
I've heard that you should run a Q-switched ND:YAG laser at its maximum energy q-switch (i.e. for our laser it is 200 uS as opposed to 400 uS) and then knock down the energy with an ND filter as...
12 June 2016 4,187 17 View
I have some samples of frozen PBMCs (peripheral blood mononuclear cells) that I am running through a flow cytometer setup. The cells are frozen using a standard DMSO based protocol. When...
29 April 2016 3,517 2 View
I'm looking for the least expensive picosecond laser with the following minimum specifications. Ideally, my upper end in cost would be ~$10,000 so anything around that or less would be...
05 March 2016 4,188 4 View
I'd like to create a photoacoustic system that uses a very inexpensive 0.1 mJ per pulse nanosecond (pulse duration) laser. However, these lasers are typically very expensive (>$10k). The best...
30 January 2016 8,073 7 View
How do I coat glass with streptavidin? (a written protocol would be nice if available)I'm trying to make a system that can detect a biotin based marker in an ELISA like setup, however, I can't use...
09 November 2015 899 4 View
Why is DAPI counterstaining used AFTER all other antibodies are attached to cells during immunohistochemistry protocols?I ask because for my particular protocol, it would be easier for me to do...
27 June 2015 3,945 4 View
I am attempting to create a new form of microscopy, however, in order to do this I need a TIRF objective (any TIRF objective that actually can cause TIR). I could try to create a setup to do this...
06 April 2015 3,118 2 View
I'm looking for a decent system to transfer cancer cells (specifically HS936 cell line melanoma) from one dish to another using a micromanipulator setup.In the past I have used this...
19 December 2014 3,421 1 View
I contacted Fisher Scientific about what the flatness of their fisherfinest microscope slides is, and they told me that they do not know that information.I have deposited some nanometer films on...
31 October 2014 1,195 3 View
