7 Questions 8 Answers 0 Followers
Questions related from Benjamin Samuel Goldschmidt
I've heard that you should run a Q-switched ND:YAG laser at its maximum energy q-switch (i.e. for our laser it is 200 uS as opposed to 400 uS) and then knock down the energy with an ND filter as...
12 June 2016 4,146 17 View
I have some samples of frozen PBMCs (peripheral blood mononuclear cells) that I am running through a flow cytometer setup. The cells are frozen using a standard DMSO based protocol. When...
29 April 2016 3,474 2 View
I'm looking for the least expensive picosecond laser with the following minimum specifications. Ideally, my upper end in cost would be ~$10,000 so anything around that or less would be...
05 March 2016 4,147 4 View
I'd like to create a photoacoustic system that uses a very inexpensive 0.1 mJ per pulse nanosecond (pulse duration) laser. However, these lasers are typically very expensive (>$10k). The best...
30 January 2016 8,024 7 View
How do I coat glass with streptavidin? (a written protocol would be nice if available)I'm trying to make a system that can detect a biotin based marker in an ELISA like setup, however, I can't use...
09 November 2015 856 4 View
Why is DAPI counterstaining used AFTER all other antibodies are attached to cells during immunohistochemistry protocols?I ask because for my particular protocol, it would be easier for me to do...
27 June 2015 3,906 4 View
I'm looking for a decent system to transfer cancer cells (specifically HS936 cell line melanoma) from one dish to another using a micromanipulator setup.In the past I have used this...
19 December 2014 3,372 1 View
