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Questions related from Behroz Khan
I am working on Thalassemia patients and want to check their hypoxia status by expression analysis of the concerned genes. Could anyone recommend which gene would be more suitable in this case;...
31 January 2020 8,374 3 View
I am working on hypoxia induced lipidomic changes in leukemia cell line. I induce hypoxia by CoCl2. I want to know if their is an easier method (some kind of dye or a distinct morphological...
16 October 2019 7,749 3 View
I want to isolate RBC Ghosts from 2-4ml blood collected in EDTA tubes. I would be glad if someone could direct me towards a protocol regarding this. It is also to be noted that the point of my...
08 March 2019 1,212 2 View
I want to know the protocol for Isolating RBCs from whole blood collected in EDTA tubes. I am planning on studying the lipidomics of the RBC Membranes therefore need to isolate RBCs in intact form.
24 November 2018 9,166 3 View
How long RBC can remain intact in whole blood collected in EDTA Tubes before being processed for Isolation of RBCs? I want to know conditions for storage of such samples so that they can be...
24 November 2018 9,492 14 View
I have got ct values for Experimental gene in cancer samples, GAPDH in cancer samples, Experimental gene in control and GAPDH in control.Now I know about the double Delta Ct method. We will get...
10 August 2017 1,574 8 View
I am doing expression profiling of different miRNAs in breast cancer patients. I did qpcr analysis using GAPDH as housekeeping gene and got GAPDH values that were varying considerably. Mostly they...
07 August 2017 6,370 3 View
Can RNA later be used to preserve whole blood samples in order to keep the RNA intact? If Yes, For how long can the RNA remain intact in whole blood samples preserved using RNA Later at room...
14 May 2017 876 3 View
