Batool Ismail

11 Questions 11 Answers 0 Followers

Questions related from Batool Ismail

Is there any good kit or method to clean DNA and RNA after extraction?

Hello there! I wonder if there is a good way to recover DNA and RNA extracted samples which are with low purity? Thanks in advance.

27 November 2022 1,499 2 View

What are the best concentration and volume for Pyro-sequencing?

Hi there, I'm preparing my DNA samples for Pyrosequencing outside of my country so i'm wondering about concentration and volume that should be been in DNA samples before I send them? Thanks in...

28 December 2021 2,572 2 View

Has anyone worked with Absolute qPCR quantification for bacterial genome?

Hello there! Does anyone have experience with absolute quantification using SYBR green kit for bacterial dna ? And plasmid as positive control, Could tell me the steps and his advice that i have...

06 August 2021 8,505 6 View

Why do we fix the RNA concentration before cDNA that will be used in qPCR next ?

Hi! I wonder about total RNA concentration that is fixed before cDNA and qPCR. so why we do that since the delta delta ct will normalize the different in gene expression between samples ?

12 July 2021 876 3 View

Could i transfer cell culture from 12 wells plate ?

hi all! Could I transfer my cell culture I've already seeding in 12 well plate and treaded with some reagents in CO2 incubator, into 96 well plate to read the results by plat reader which couldn't...

24 May 2021 7,435 2 View

How could we measure the total miRNA after extraction ?

Hi there! we are trying to measure the concentration of miRNA, after using miRNA extraction plasm/serum kit (Qiagen), by nano drop but the concentration is very low, so any one has an experience...

21 March 2021 9,041 5 View

Why does the DNA's elution decrease after centrifuging with spin column?

Hi there! Has anyone faced this problem, when i add the elution buffer to spin columns then centrifuge them I didn’t get the same volume as which I added, why ? does that relate to increasing the...

03 January 2021 1,147 4 View

RBC's contamination during DNA extraction ?

Hi there! currently i am working with spin column kit for DNA extraction I've put almost 600 uL of buffy coat. after a lot of washing, the RBC's remained stucking in filter? How could i get rid...

26 October 2020 8,884 4 View

Which is the best for RNA extraction is it from whole blood or PBMC?

hello their! I've to extract RNA of specific protein from blood using Zymo Research kit to investigate the expression of that protein, so would any one please tell me which way is best for extract...

21 August 2020 8,615 3 View

Which DNA methylation kit is the best for use ?

Hi all! am gonna to study the methylation status for target promoter gene . I have seen that there are alot of kits can be used such as Zymo kits and Qiagen kits but which one is the best ,also...

14 March 2020 6,835 6 View

How i can determine Gene promoter and CpG islands?

Hi ! i will work on specific gene and i have to search about methylation status for it , so how i can determine the promoter for this gene ? And define how many CpG islands there are or in total...

27 February 2020 9,349 2 View