Barbara Loi

7 Questions 15 Answers 0 Followers

Questions related from Barbara Loi

Anyone who can share a tested hematoxylin/eosin protocol on samples included in methacrylic resin?

Hi everyone, I am cutting fish larvae after their embedding in Technovit 7100. I usually use methylene blue/azure II/basic fuchsin in the staining phase. However, I noticed that for some tissue...

18 November 2021 1,512 3 View

Any suggestions to avoid the break of resin slices (Technovit 7100) on the microtome blade?

Hi everybody, I am beginning with the inclusion of biological samples (fish larvae) in resin (i.e. Technovit 7100). I am experimenting some troubles during the cut at the microtome (I would like...

17 November 2020 2,550 3 View

Methylene blue/azure II/basic fuchsin stain for resin section: is possible to substitute methanol in the recipe?

Hello, I need to stain my fish larvae sections (embedded in Technovit 7100 resin) with the methylene blue/azure II/basic fuchsin stain. I have this recipe: Polychrome I 0.325 g Methylene...

05 March 2020 7,306 13 View

Which is the storage temperature of glutaraldehyde (grade II, 25% in H2O)?

Hi everybody, anyone who knows which is the right storage temperature for glutaraldehyde (grade II, 25% in H2O)? It is not for electronic microscopy, but only for fixation of histological samples...

21 January 2019 7,767 14 View

Any available protocol for fish larvae histology?

Hello everybody, I have been working for several years on sea urchin gonad development using histological techniques, now I am going to start working on the histology of fish larvae (Pagrus pagrus...

27 April 2018 1,957 8 View

How can I label similar groups in a bar graph?

Hi everybody, I need to examine the seasonal variation of the gonadosomatic index of the sea urchin Paracentrotus lividus. I have the following post-hoc results (SNK-test): Autumn = Summer Summer...

01 December 2017 1,856 12 View

Hi everybody, is the transitivity a valid concept in statistics?

I mean, I have a=b, a=c but b≠c. Do I have to to consider b=c?

03 August 2017 7,171 3 View