Barbara Bartolini

2 Questions 2 Answers 0 Followers

Questions related from Barbara Bartolini

How to digest a plasmid with sequential restriction enzyme sites?

Hello, I have two sequential restriction enzyme sites I have to use: CTCGAGTCTAGA (respectively Xho I and Xba I). They perform well in the same buffer, nevertheless I suspect my digestion is not...

12 October 2018 5,703 1 View

Which markers for S-G2 phase should I check for cell cycle analysis with FACS?

I need to stain cell populations to check the progression through different phases of cell cycle. Which are the usual markers I should check by FACS?

11 December 2013 4,855 2 View