Does anyone simulate the supercooling phenomenon of the PCM (COMSOL)?

I have a heat transfer model in the thermal bath (temperature changes in the range 23-33degC), in which a vial with PCM material is immersed (melting temperature is 28degC). During the heating...

03 January 2021 3,548 2 View

My Wright-Giemsa Stain on macrophages isn't showing any cytoplasm, just nucleus. Any tips to improve my staining?

I'm pretty sure they are mostly macrophages since after a 2 hour incubation in M199 they are stuck with pseudopodia. My slides just aren't pretty enough for me. I'm using Fisherbrand Wight-Giemsa...

13 December 2020 5,475 3 View

Any suggestions to avoid the break of resin slices (Technovit 7100) on the microtome blade?

Hi everybody, I am beginning with the inclusion of biological samples (fish larvae) in resin (i.e. Technovit 7100). I am experimenting some troubles during the cut at the microtome (I would like...

16 November 2020 2,373 3 View

How to obtain promoter sequences?

Hi dear fellows, I would like to perform ChIP-PCR on the promoter sequences of genes, but I am not quite sure how to design primers for the promoter sequences. Can someone please help me? My...

20 September 2020 7,371 6 View

Recommended packages or references to combine regional climate models and Holdrige life zones with R?

I want to use R to make regional climate models to analyze changes in Holdridge's life zones. I haven't used R in a long time so I'm kind of outdated and would like to read some references that...

01 July 2020 1,728 4 View

What strategies to use to make distance classes more attractive to students?

In times of pandemic, distance learning has gained strength. What can teachers do to make distance classes more attractive to students?

23 May 2020 8,148 22 View

In your opinion, are bibliographic review articles important and should they be published by scientific journals?

Some journals do not accept review papers because they consider that the theoretical framework is the basis of a research, and not the research itself. Other magazines already accept and encourage...

20 May 2020 7,841 50 View

Metabarcoding fungi in lake sediment cores - advantages/disadvantages of ITS and 18S?

In my PhD, I want to analyse fungal communities in lake sediment cores from Siberia. One of the performed experiments will be a metabarcoding of the fungi. Therefore I am evaluating primers at the...

05 May 2020 3,920 1 View

Which species do you recommend for ecotoxicological tests of industrial effluents containing hydrocarbons?

The question aims to know what types of species are most used in ecotoxicological tests of effluents from industries in the oil sector.

26 April 2020 5,382 21 View

How can I include a second order term in the model?

My hypothesis is that the functional diversity of fish (responsible variable) increases until an optimum level of a gradient of habitat structural complexity (predictor variable), but decreases...

01 April 2020 9,569 4 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

Why cloning of gene into pCAMBIA1304 vector is unsuccessful?

I have tried to clone a specific gene into pCAMBIA1304 vector. But all the attempts failed. I used ligation kit from both Promega Corporation and Takara Bio. However, there was one time I used...

04 February 2021 525 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View