Banyar Than Naing

6 Questions 17 Answers 0 Followers

Questions related from Banyar Than Naing

Is there anyone who knows the mouse testis specific lnc RNAs?

I am finding long noncoding RNA and mRNA related specific to mouse testis. If anyone knows very well about the biomarkers like Insulin-like factor 3 (INSL3) which is specific to testis, please let...

09 September 2014 1,165 2 View

What are the advantages and disadvantages of using Standard curve in stead of delta delta Ct method in Real Time Gene expression assay?

When we want to assess the gene expression assay by Real Time PCR, there are mainly Taqman gene expression assay or SYBR green gene expression method in which we need to set up the primer set by...

06 June 2014 3,085 5 View

How can you study easily to understand different RNAs like coding RNA, non coding RNA?

Since there are so many different types of RNAs, snRNA, siRNA, miRNA, rRNA, tRNA, mRNA, I would like to know how to study all these RNAs easily.

03 March 2014 2,427 4 View

How do you differentiate whether nucleotide changes causing amino acid changes is mutation or single nucleotide polymorphism?

In our laboratory, I am analyzing genetic diseases related genes. Sometime, I found nucleotide changes causing amino acid changes but it was not contained in the reported SNP list of NCBI....

03 March 2014 3,894 12 View

What is the best calculation method to compare the data between Taqman Real time PCR and Taqman Digital PCR?

I would like to compare the data between Taqman Real time PCR and Taqman Digital PCR using 6 samples and 8 target genes. I want to know whether delta delta Ct is ok or not for comparison. I also...

10 October 2013 4,947 7 View

Which one is better for RT-PCR and making cDNA, using Random primer or Oligo dT primer or both primers?

I want to know the difference between Random primer and Oligo dT primer. Do we need to use the kit containing both Random primer and Oligo dT primer?

03 March 2013 5,477 1 View