Bahareh Khalaj

5 Questions 15 Answers 0 Followers

Questions related from Bahareh Khalaj

can I used blue laser 488nm for Antibody which has emission at 510?

Hi. I have one Antibody which have EX430 and EM510 and I have access to Accuri flowcytometer which have blue lase and red laser? now my question is can I used this AB with this flowcytometer or...

07 November 2019 8,834 4 View

Which marker is positive for Human Microglia cell line from ATCC (HMC3) by FACS?

HI every one, I runned FACS on HMC3 and analyse them but I didnt get any positive marker after activation with IFN gamma and LPS. According to ATCC , HMC3 should be postive for CD14 at resting...

22 May 2018 6,520 17 View

2mM EDTA can detach Mesenchymal stem cells from cell culture plate?

I co culturing PBMC and MSC cells. PBMC become so adhesive efter 72 hour incubation. IF I used PBS with 2mM EDTA, can Mesenchymal cells detach too? I dont want mesenchymal stem cells detach! has...

03 May 2017 1,224 2 View

Why PHA-M doesn't increase PBMC population and prolifration after 72H?

I tried to do proliferation assay with PBMC and MSC. I have followed cells proliferation with CFSE. I activate PBMC with PHA-M(2,5microg/ML) and I labeled PBMC with 1microM CFSE But after 72 h,...

28 April 2017 5,644 1 View

What is the lowest concentration of CFSE which I can use for PBMC proliferation assay?

I tried to do proliferation assay on activated PBMC but it looks like CFSE kills all my cells. I tested 10 micro M and 5 microM concentration of CFSE but none of them worked. can anybody help me...

20 April 2017 7,956 5 View