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Questions related from Ashish Saroha
Dear all, I want to inject the FACS sorted iNKT cells into the mice liver. I am thinking of injecting retro-orbitally but I don't know how many cells will eventually reach the liver. Moreover, I...
07 July 2016 1,453 2 View
I want to check the distribution of some proteins in the hepatocytes and ECM of liver by Western blotting. Thanks
02 February 2015 8,145 11 View
Hi, I am facing some problems in growing HelaS3 and K562 cells in suspension. Firstly, the media starts turning acidic (yellow) even at very low density of the cells i.e. ~0.3 million cells/ml....
07 July 2013 6,704 2 View
I want to cross-link the TNF receptor with His or flag tagged TNF using chemical reversible or irreversible cross linkers. After Incubating for two hours on ice with 200ng/ml TNF with 50 million...
02 February 2013 8,945 2 View
My pET28 clone containing 3 flag-Avitag and TNF alpha fusion protein is not expressing in E.coli Bl21 (DE3) cells. Sequencing results of the clone showed that all the sequences are in frame.
01 January 2013 6,325 9 View
