Ashish Bhandari

8 Questions 10 Answers 0 Followers

Questions related from Ashish Bhandari

What are the genetic markers that can be tested for characterizing Brain Tumors (Gliomas and Meningiomas) using using routine PCR and/or blotting?

We are trying to look for potential genetic markers that can be used to diagnose and type different types and grades of gliomas and meningiomas in brain tumor patients. We also have a recurring...

30 November 2014 962 0 View

Which gene should I consider when studying the mutation prevalence in sickle cell anemia? What are the restriction enzymes that can be used?

We are trying to detect the prevalent mutation in case of sickle cell anemia in a specific population. We are confused about which gene we should consider as we were unable to find much literature...

14 November 2014 3,050 7 View

Can anyone provide me with the Salmonella typhimurium strains for Ames test?

We were planning on conducting a study on possible suspected mutagens but are stuck due to budgetary constraints and the unavailability of the strains locally.

11 September 2014 4,649 5 View

When performing a BLASTn result interpretation, what should the main priority be?

When I was performing BLASTn, the result shows multiple entries with 99-100% query coverage and 99-100% identity. In such cases should I select the entry with the max score? Also in some cases the...

02 July 2014 6,484 2 View

Can I prepare a single phylogenetic tree using sequences from two different loci?

We have sequenced two different loci (ITS and rbcl) and I was wondering if I could combine both loci and build a single phylogenetic tree.

26 June 2014 2,815 8 View

Why and how does PEG precipitate DNA? Why is it better than isopropanol to reduce the polysaccharide contaminants?

We used to perform isopropanol precipitation of DNA isolated from plant tissues but had a lot of polysaccharides as contaminants and while going through different literatures we found the use of...

14 March 2014 9,038 2 View

Is it possible to isolate Thermus aquaticus from native soil samples?

We were trying to isolate some thermophilic bacteria when the idea suddenly struck us to isolate Thermus aquaticus from hot springs. But before approaching hot spring sources we wanted to try...

16 November 2013 3,534 4 View

How to prevent the drying and evaporation of culture media when incubating at higher temperatures?

While trying isolation of thermophiles, we came across a difficulty: when we incubated our sample at 50 degree celsius, almost most of the media dried up. How do we prevent the drying up of...

16 November 2013 7,927 17 View