Artur Ribeiro

5 Questions 8 Answers 0 Followers

Questions related from Artur Ribeiro

Can i use other amino acid in C-extein rather than Ser, Thr or Cys?

Hi all, i'm thinking in taking advantage of inteins to produce a specific peptide. I'll clone my peptide as the C-extein of the construct. However, i've a doubt regarding the first amino acid...

01 January 2018 2,897 0 View

There is any good review about the loss of activity during enzyme immobilization and how to solve it?

I would like to know the best approaches for enzyme immobilization in order to avoid/solve the loss of enzyme activiy during immobilization. I've already try encapsulation but at the end of one...

01 January 2015 2,744 7 View

How can I quantify the number of free thiol groups on small peptides?

I want to measure the number of free thiol groups on small peptides. My first approach was the Ellman's reagent, however I want to check other approaches.

09 September 2014 5,925 2 View

Does a protei produced in Saccharomyces is always glycosilated?

I want to check if a protein produced in Saccharomyces is always glycosilated. And if so, it could display an immunlogical reaction in humans?

09 September 2014 3,085 8 View

There are any optimized protocols to modify proteins with Isothiocyanate derivatives?

I'm trying to modify an enzyme with several Isothiocyanate derivatives. I want to use an approach that does not compromise the enzymes activity.

06 June 2014 8,728 3 View