I want to measure the number of free thiol groups on small peptides. My first approach was the Ellman's reagent, however I want to check other approaches.
Gerardo Ferrer-Sueta
Ellman's is the most popular by far, but I prefer 4-dithiodipyridine, which is faster and more sensitive. Here is a comparison of both methods.
http://www.ncbi.nlm.nih.gov/pubmed/?term=12110978
There is a modified method for enhanced sensitivity here
http://www.ncbi.nlm.nih.gov/pubmed/?term=17286954
I use the simple version practically on a daily basis and it works great. Of course there are many other methods for quantitating thiols.
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