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Questions related from Arpit Shukla
During installation of LC-MS instrument, vacuum is required in time-of-flight (ToF) tube. A value of 1.3 x 10^-7 is suggested as per the Company. They say a minimum of 10 days is required to...
24 August 2021 7,451 4 View
We have a set of probes for which we need to check if the probe has non-specific targets. But we need to search each probe against a large number of genomes. A large number of hits are coming up...
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A protein undergoes reduction in its fluorescence intensity upon ligand binding. Upon titrating with the ligand, can we find the number of identical binding sites in a multimeric protein ? For...
09 March 2017 3,858 1 View
Is there a standardised protocol available for this method? Has anyone tried it with samples containing nucleotides or proteins? I already have reference paper but insufficient information. Ref 1...
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Fragment size is 1-1.5 kb. Sample is already cleaned-up before restriction digestion. I just want to remove the enzyme before using the sample further for ligation.
26 August 2015 7,939 3 View
Upon mutating a tryptophan to a non-aromatic residue, if there is a blue shift in the intrinsic protein fluorescence emission spectra, what does it suggest? Note: The protein may contain 2 or more...
06 July 2015 6,010 0 View
I need to use TCA to deproteinize my samples. Genesis C18 columns can withstand upto 1 pH but my concern is the corrosive nature of TCA. Is it safe to use TCA (max. 12%) with this column?
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The enzyme forms inosine and inorganic phosphate (Pi). Some kits are available which contain enzyme PNP that uses substrate MESG and Pi to give some colored product. However that may not work for...
01 January 1970 282 2 View
