Arpit Shukla

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Questions related from Arpit Shukla

How much time does it take to achieve a vacuum of 1.3 x 10^-7 in ToF tube in LC-MS?

During installation of LC-MS instrument, vacuum is required in time-of-flight (ToF) tube. A value of 1.3 x 10^-7 is suggested as per the Company. They say a minimum of 10 days is required to...

24 August 2021 7,434 4 View

How do we check for non-specific targets in microarray probes for genomes other than the host?

We have a set of probes for which we need to check if the probe has non-specific targets. But we need to search each probe against a large number of genomes. A large number of hits are coming up...

25 May 2021 3,855 4 View

How do we determine the number of identical ligand binding sites in a protein based on a fluorescence quenching assay ?

A protein undergoes reduction in its fluorescence intensity upon ligand binding. Upon titrating with the ligand, can we find the number of identical binding sites in a multimeric protein ? For...

09 March 2017 3,848 1 View

Can I use TCA with Genesis C18 4um column?

I need to use TCA to deproteinize my samples. Genesis C18 columns can withstand upto 1 pH but my concern is the corrosive nature of TCA. Is it safe to use TCA (max. 12%) with this column?

28 October 2013 3,183 0 View

How do I perform continuous measurement of nucleotidase activity ?

The enzyme forms inosine and inorganic phosphate (Pi). Some kits are available which contain enzyme PNP that uses substrate MESG and Pi to give some colored product. However that may not work for...

01 January 1970 272 2 View