Arindam Pal

4 Questions 2 Answers 0 Followers

Questions related from Arindam Pal

Wave of calcium coming during Fluo4-AM imaging with endothelial cells?

I am trying to monitor calcium signals with ATP and TRPv4 in endothelial cells (bEnd.3). For this i incubate my cells with 3-5uM Fluo-4AM in 1X HBSS (pH 6.8).after 30mins, i wash with HBSS twice...

4 weeks ago 895 1 View

How important is flow for ca2+ analysis in cells in response to a drug?

Hi, I am studying the Ca2+ response on hCMEC/D3 cells and have been adding my drug of interest with a pipette (at the speed of about 50-100uL/sec). However this addition is not in control and I...

11 July 2023 7,449 0 View

Differences in culture media for in vitro blood-brain barrier model?

We are using the Endogro-SCME004 medium for cultivation of hCMEC/D3 cells, but since it is out of stock for a while, we decided to switch to Lonza. However, we just realized that there are stark...

22 June 2023 5,131 0 View

How do I learn to do in silico analysis?

I want to see the change in binding ability of the enzyme with the substrate in presence of a probable drug molecule insilico. Where can I find reliable information to learn about the basics from...

02 February 2019 7,650 4 View