Apoorva Verma

9 Questions 11 Answers 0 Followers

Questions related from Apoorva Verma

Hetero (Flox/WT) with Krasg12D mice become Sick?

Hi Everyone, I am encountering a strange problem in mouse lung cancer studies I have 2 sets of mice: 1. flox/flox +KrasG12D with Cre (Homo Test) 2. flox/WT + KrasG12D with Cre (Hetero Ctrl) I...

04 April 2019 2,154 2 View

DNA isolation from mouse lungs?

Dear all, I want to isolate DNA from mouse lungs to check for gene deletion (The Cre is expressed specifically in Type II alveolar cells). I tried to isolate the DNA using the method discussed...

02 February 2019 2,793 6 View

Tamoxifen injection causes abortion in mice or affects second pregnancy??

Hi all, I use Tamoxifen inducible cre (cre/ERT2) system to knockout a gene at embryonic day (E12.5) by injecting 1.2mg tamoxifen in 100ul corn oil. (injection vol = 100ul) First time, the females...

09 September 2018 9,582 0 View

Difference between U6 and H1 promoter for shRNA?

Hi, I made a construct using mouse specific shRNA driven by U6 promoter, it did not show the knockdown of my target gene in NIH/3t3 cells. Same shRNA sequence used by another group, works in...

12 December 2017 3,990 5 View

Problems in Rescuing phenotype?

Hi all, I am struggling for sometime to understand how rescue works. In my case, I kd (knockdown) N-WASP (actin regulating protein) in HaCaT cells using shRNA based Lentivirus mediated...

01 January 2017 7,160 3 View

Suggestions for Good Ras antibody?

Hi all, I am overexpressing mutant kras(g12d) in NIH3t3 cells and selecting with Neomycin (my construct is kras(g12d) in pLJM-linker with Neo res gene). After complete selection when uninfected...

12 December 2016 8,696 3 View

Keratinocyte media for HaCaT for organotypic culture?

HI I need some advice regarding media for keratinocytes for organotypic culture. I am using HaCaT to develop organotypic culture. My HacaT are cultured and grown in high glucose DMEM + 10% FBS...

11 November 2016 5,813 7 View

Protein incubation with mammalian cells for MTT assay?

Hi everyone.. A brief intro first I am trying to do one assay with my peptide conjugated with GFP and the control is GFP. I purified both proteins using Ni-NTA as they possess His-TAG. The purity...

07 July 2016 3,871 2 View

Query on green auto-fluorescence in A549 & HEK-293T cells?

I have been working with A549 & HEK-293T cells and trying to do protein localization studies. I have conjugated my protein with His-GFP and the control is only His-GFP. I do find GFP signal in...

03 March 2016 6,973 5 View