Apoorva Verma

6 Questions 7 Answers 0 Followers

Questions related from Apoorva Verma

DNA isolation from mouse lungs?

Dear all, I want to isolate DNA from mouse lungs to check for gene deletion (The Cre is expressed specifically in Type II alveolar cells). I tried to isolate the DNA using the method discussed...

13 February 2019 5,182 5 View

Pore size of culture inserts for organotypic culture

Hi all I would like to know the appropriate pore size culture inserts to be used for skin organotypic cultures. In many papers they mention the use of 6 well inserts with 3um pore size while few...

12 June 2017 6,034 3 View

Difference between FBS and FCS?

Hi I want to know whether there is any difference between FBS (fetal bovine serum) and FCS (fetal calf serum). Most people say it is the same thing. However, I plan to culture NIH/3T3 cells and...

29 March 2017 6,111 14 View

Suggestions for Good Ras antibody?

Hi all, I am overexpressing mutant kras(g12d) in NIH3t3 cells and selecting with Neomycin (my construct is kras(g12d) in pLJM-linker with Neo res gene). After complete selection when uninfected...

05 December 2016 5,681 2 View

Foci formation assay in NIH3t3 cells?

Hi, I am having some trouble to do Focus formation assay in NIH3t3 cells. I start with making NIH3t3 cells stable using retrovirus (90% infection efficiency after 72hrs with pSIR-GFP virus). My...

31 October 2016 868 2 View

Protein incubation with mammalian cells for MTT assay?

Hi everyone.. A brief intro first I am trying to do one assay with my peptide conjugated with GFP and the control is GFP. I purified both proteins using Ni-NTA as they possess His-TAG. The purity...

07 July 2016 8,914 2 View