11 Questions 15 Answers 0 Followers
Questions related from Apoorva Verma
Dear all, I want to isolate DNA from mouse lungs to check for gene deletion (The Cre is expressed specifically in Type II alveolar cells). I tried to isolate the DNA using the method discussed...
13 February 2019 5,191 5 View
Hi I am studying the role of my protein in lung cancer and using A549 (epithelial lung adenocarcinoma) and H1299 (Non small lung cancer ). Could someone please suggest what would be the most...
17 April 2018 4,370 3 View
Hi all I would like to know the appropriate pore size culture inserts to be used for skin organotypic cultures. In many papers they mention the use of 6 well inserts with 3um pore size while few...
12 June 2017 6,041 3 View
Hi I want to know whether there is any difference between FBS (fetal bovine serum) and FCS (fetal calf serum). Most people say it is the same thing. However, I plan to culture NIH/3T3 cells and...
29 March 2017 6,118 14 View
Hi Iam trying to make NIH3t3 mutant kras stable cells.For this, I have made kras(g12d) construct in pSIR neo vector. I infected nih3t3 cells using retrovirus and selected with neomycin for 5 days...
10 December 2016 345 1 View
Hi all, I am overexpressing mutant kras(g12d) in NIH3t3 cells and selecting with Neomycin (my construct is kras(g12d) in pLJM-linker with Neo res gene). After complete selection when uninfected...
05 December 2016 5,687 2 View
I am trying to make 10X hanks balanced salt (HBS) with phenol red. I have HBS powder and was planing to make 10x solution out of it. I wanted to ask: should I supplement 10X HBS solution with 10...
23 November 2016 7,250 3 View
Hi all, I am trying to develop 3D collagen gel embedded with fibroblast. I came across few protocols in which they add 10X MEM to collagen solution (acts as pH indicator) and after which few drops...
15 November 2016 3,997 10 View
Hi, I am having some trouble to do Focus formation assay in NIH3t3 cells. I start with making NIH3t3 cells stable using retrovirus (90% infection efficiency after 72hrs with pSIR-GFP virus). My...
31 October 2016 930 2 View
Hi everyone.. A brief intro first I am trying to do one assay with my peptide conjugated with GFP and the control is GFP. I purified both proteins using Ni-NTA as they possess His-TAG. The purity...
07 July 2016 8,925 2 View
I have tried lentivirus which showed poor GFP expression. We do not have sarcoma or adenovirus system. the other alternative is retrovirus, has anyone done infection of mouse 3t3 using...
06 January 2016 2,362 5 View
