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Questions related from Anuroop Venkateswaran Venkatasubramani
Hi all.. I have been quite confused with statistical tests.. I think my doubt is very preliminary but I am not sure.. I have two populations (treated and un-treated) each with n=3. I would like to...
28 June 2015 3,530 40 View
Hi all, I am doing qPCR for my stable mouse cell line and I am using 18S rRNA as an internal control. The Cp values of my test samples (RT-ve) were nil, i.e. no Cp value, but in the case...
15 April 2015 7,738 28 View
Hello guys!! I just have a doubt and I hope someone could give me an explanation. I have been doing lentiviral transduction and lipofection for quite a while. In both cases, absence of serum is...
25 March 2015 10,088 6 View
I am using two different systems for my RNAi screen and both of them seem to have 2 origins of replication and I am not clear what the reason is? One of them has a f1 and pUC ori and the other has...
07 March 2015 2,786 6 View
Hi all, dor lentiviral packaging, I used both HeLa and HEK cells and I found out that packaging had occurred in HEK but not in HeLa. Is there any particular reason for that? Has anyone tried...
21 November 2014 5,344 7 View
Hi, I have started to optimize my cell line for Lentiviral transduction and I found that my cells were positive for mycoplasma contamination again although this was a new batch of cells. I will be...
20 October 2014 9,102 10 View
I have been using mouse mammary adenocarcinoma cells and I have recently observed black dots. I was doing Puromycin-kill curve and so the cells were treated with Puromycin. I am not sure if the...
03 October 2014 9,788 4 View
I had used Permutation in Plink to check the validity of my markers after 10000 permutations. I used the command --mperm 10000 for permutation. Is there a way to get the Standard Deviation of the...
07 March 2013 2,017 7 View
