Anuroop Venkateswaran Venkatasubramani

9 Questions 44 Answers 0 Followers

Questions related from Anuroop Venkateswaran Venkatasubramani

Minimum sample size for t-test??

Hi all.. I have been quite confused with statistical tests.. I think my doubt is very preliminary but I am not sure.. I have two populations (treated and un-treated) each with n=3. I would like to...

28 June 2015 3,575 40 View

What is the probability for a complete knockdown?

Hi.. I am using mouse cell lines and shRNAs for my knockdown and I have obtained stables for my candidate genes. I performed qPCR and there was no Cp value for KD samples. I did it thrice and...

01 May 2015 5,392 9 View

Are you familiar with Cp values for 18S rRNA?

Hi all, I am doing qPCR for my stable mouse cell line and I am using 18S rRNA as an internal control. The Cp values of my test samples (RT-ve) were nil, i.e. no Cp value, but in the case...

15 April 2015 7,809 28 View

Why or how does serum reduce the transfection efficiency?

Hello guys!! I just have a doubt and I hope someone could give me an explanation. I have been doing lentiviral transduction and lipofection for quite a while. In both cases, absence of serum is...

25 March 2015 10,137 6 View

Why do most vectors have 2 origins?

I am using two different systems for my RNAi screen and both of them seem to have 2 origins of replication and I am not clear what the reason is? One of them has a f1 and pUC ori and the other has...

07 March 2015 2,869 6 View

Is there any particular reason for using HEK cells for lentiviral packaging?

Hi all, dor lentiviral packaging, I used both HeLa and HEK cells and I found out that packaging had occurred in HEK but not in HeLa. Is there any particular reason for that? Has anyone tried...

21 November 2014 5,403 7 View

Will mycoplasma infection have any effect on my lentiviral transduction?

Hi, I have started to optimize my cell line for Lentiviral transduction and I found that my cells were positive for mycoplasma contamination again although this was a new batch of cells. I will be...

20 October 2014 9,144 10 View

Is it possible to differentiate between Mycoplasma contamination and cell debris?

I have been using mouse mammary adenocarcinoma cells and I have recently observed black dots. I was doing Puromycin-kill curve and so the cells were treated with Puromycin. I am not sure if the...

03 October 2014 9,841 4 View

How to get the Standard Deviation?

I had used Permutation in Plink to check the validity of my markers after 10000 permutations. I used the command --mperm 10000 for permutation. Is there a way to get the Standard Deviation of the...

07 March 2013 2,068 7 View