Anup Kumar Singh

6 Questions 16 Answers 0 Followers

Questions related from Anup Kumar Singh

What should be the amplicon size for Chip based DNA amplification?

For my prospective Chip experiment, I am designing the primers for the promoter region of target gene to include the binding site of putative TFs in the amplicon. What should be amplicon size for...

09 September 2014 1,252 1 View

What are the symptom of Ebola virus infection? Is there any diagnostic method to detect it?

Ebola outbreak is emerging as a major challenge in many countries. I intend to ask others opinion about the threat as it’s time to be educated on its consequences — what does it mean, how does it...

08 August 2014 2,654 5 View

What you think about the expression of death receptors on cancer stem cells (CSCs) and non-CSCs within the tumor population?

Tumor heterogeneity is widely accepted at the population level within the tumor. Minor population within the tumor cells known as CSCs are thought to be highly drug resistant compare to non-CSCs....

06 June 2014 4,832 1 View

How do I dissolve tetrachloro 1,4-benzoquinone and what is an appropriate solvent for it?

I am trying to make a solution of tetrachloro 1,4-benzoquinone, but am unable to dissolve it. I tried to do so in PBS, methanol, ethanol, and DMSO, but it was not soluble in any of these solvents.

05 May 2014 2,649 4 View

Software for Affymetrix miRNA 3.0 array data analysis? What is advantage of using Gene spring and what are limitations of expression console?

I am learning Affymetrix miRNA 3.0 array data analysis? I want to know the difference between Gene spring and expression console? How do they differ in statistical analysis?

03 March 2014 9,018 1 View

What are the ways to enrich a cancer stem cell population in cell lines?

I am trying to enrich CSC population in serum free media for further analysis.

01 January 2013 7,929 5 View