Anthony Cannavicci

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Questions related from Anthony Cannavicci

Protocol for stimulating HUVECs with TFGB1?

Does anyone have a detailed protocol on stimulating HUVECs with TGFB1? I am aware that most papers use 10 ng/ml of TGFB1 with serum free media for 24 hours. Is this media without growth factors as...

23 April 2021 9,170 1 View

Can someone please confirm if they were able to detect VEGFA in HUVECs via Western Blot?

Currently using R&D pan-VEGF monoclonal antibody (MAB293-SP). This antibody works as I was able to detect purified VEGF. BACT loading control looks great, but I cannot detect any VEGF from my...

02 November 2020 6,134 2 View