Annele Sainio

4 Questions 3 Answers 0 Followers

Questions related from Annele Sainio

Which is the best method to extract vesicles from cell cultures?

I am planning to extract cell secreted vesicles from different type of cell cultures. There are some kit based methods available. However, I am wondering if the classical differential...

28 April 2020 5,726 4 View

Cytotoxicity analysis for adenovirus transduced cells?

I am studying the effects of an modified adenovirus on different cancer cells. The problem is that most cytotoxicity assays rely on the use of a spectrophotometer for measuring absorbance etc....

18 April 2018 8,757 1 View

Protocol using SONOPULS for the sonication of HUVSMCs?

I want to sonicate human umbilical vein smooth muscle cells troughly. Cells are grown to confluent in 6-well plates, scraped and sonicated. How long (s)? Cycle? And what power?

16 February 2018 1,617 0 View

How to conduct protein extraction of secreted ECM proteins from soft agar assays?

Hello, I am planning soft agar assay with MCF-7 cells. The cells will be transduced with an adenoviral vector before the assays and then cultured on soft agar. We are studying one of the secreted...

18 April 2016 5,787 1 View