Anna M. Czarnecka

6 Questions 4 Answers 0 Followers

Questions related from Anna M. Czarnecka

What is a suitable IGF free medium/serum?

I would like to exclude the effect of IGF (among other GF) on cell activity.

12 April 2015 5,636 4 View

Which liquid nitrogen generator would you recommend (see below)?

For in-house usage for medium size lab? Elan2? or some other? http://www.elan2.com/media/pdf/elan2ProductBrochure.pdf

14 September 2014 8,884 1 View

What is the best treatment option for patients with ccRCC and CML?

Would you combine imatinib with TKIs or mTORI?

21 June 2014 9,705 3 View

How can I evaluate TKI resistance in cell culture?

What are kits/assays to evaluate resistance of cells in culture to TKI (including sunitinib, sorafenib, gefitinib etc.) in cells that are primarily resistant to classical chemotherapy and express...

11 May 2014 1,916 2 View

Why might my MTT and AlamarBlue results be cotradictory?

Cancer cells were treated with conditioned media. In the MTT curves it shows inhibitory effect while cells treated in AlamarBlue are proliferating faster (the same cell line, the same conditioned...

14 April 2014 1,146 5 View

To compare the toxicity of anti-cancer compounds should I use the same seeding number of cells or adjust the number for each cell line?

I would like to compare the effect of 4 different TKIs on 20 different cells lines. Should I adjust the seeding cell number for each cell line (based on its growth curve) or should I start with...

14 April 2014 4,797 3 View