Ankita Dutta

7 Questions 15 Answers 0 Followers

Questions related from Ankita Dutta

I am performing tryptophan fluorescence quenching experiments for a protein and however quenching after adding inhibitor to the protein?

My protein shows intrinsic tryptophan flourescence. I used this property to study the lifetime properties. In the same context i performed acrylamide and KI based quenching methods to study...

12 December 2017 8,444 5 View

I am trying to get aclone for my 1 kb insert into a 4.5Kb vector. The colony PCR results are positive but I cant see fallout?

Insert size: 1Kb Vector : 4.5 Kb Restriction enzyme sites : NdeI XhoI Backbone was dephosphorylated with rSAP for 1 hr 37C heat inactivated later, PCR product was gel eluted after digestion from...

04 April 2017 1,470 4 View

What are the coordinates for cobalt(II) and manganese(II) available for using for MD simulation?

I want to try Molecular dynamics simulation of my protein with manganese(II) and Cobalt(II) ions. Can anyone tell me if the coordinates for the metal ions available for carrying out the same?

10 October 2015 4,807 7 View

How might I increase protein yield and solubility for helicobacter pylori recombinant proteins?

I am trying to express and purify a helicobacter protein. I had tried cloning and expression with CT his tag(pET21c+) but the protein was in hard inclusion bodies. So I changed my tag to GST...

08 August 2015 8,021 6 View

How do I increase protein solubilization from inclusion bodies?

I have a his tag protein in inclusion bodies that i purify in denatured state by affinity chromatography. I have standardized that 8M urea with or without DTT is giving me maximum solubilization...

11 November 2014 2,533 17 View

Can anyone suggest how to proceed with some molecular biology, cloning?

I have eluted plasmid backbone after double digestion using Qiagen spin column. When I ran my samples on the agarose gel I could see a single band of intensity almost comparable to 500 bp ladder...

07 July 2014 6,949 6 View

How can one find which kinase causes phosphorylation of a prokaryotic protein with no reported sites?

I ran a search for possible phosphorylation sites using online softwares. But how do I go ahead to predict which kinase may cause phosphorylation?

12 December 2013 3,008 4 View