Andrew S Lee

5 Questions 6 Answers 0 Followers

Questions related from Andrew S Lee

Can I do DiI labeling in adult mouse brains that have been kept in 1X PBS (with sodium azide)?

I want to use DiI (or other dyes that work in postmortem tissue) to trace inputs to the striatum in adult mouse brains (mainly cortico-striatal and thalamo-striatal circuits). The brains have been...

11 November 2021 1,277 2 View

Is prenatal oral CNO treatment possible?

Hi DREADD users, I want to treat CNO to pups that have inhibitory DREADDs through the maternal milk during the first postnatal week. Is it possible for CNO to get delivered via milk? And if so,...

13 December 2017 939 1 View

Why are there two bands for spinophilin western blot in rat prefrontal cortex?

Hi all,I recently ran a western blot using medial prefrontal cortex samples from PN17 rats. I followed the same protocol that other students have used in our lab (we prepare samples with RIPA...

02 November 2016 2,288 2 View

Fresh frozen cryostat section immunohistochemistry?

Hi all, I sectioned fresh frozen brains (CO2 euthanized rats) using a cryostat. I typically do immunohistochemistry with perfused free-floating brains, but I want to try it with fresh frozen...

03 August 2016 9,159 13 View

Has anyone traveled with brain sections (already coverslipped) internationally?

Hi all, I have brain sections that have been stained and coverslipped and mounted in the US. I want to scan these brains using a scanner in Germany, and I was wondering how people usually bring...

16 September 2015 1,092 11 View