Andrew S Lee

3 Questions 6 Answers 0 Followers

Questions related from Andrew S Lee

Can I do DiI labeling in adult mouse brains that have been kept in 1X PBS (with sodium azide)?

I want to use DiI (or other dyes that work in postmortem tissue) to trace inputs to the striatum in adult mouse brains (mainly cortico-striatal and thalamo-striatal circuits). The brains have been...

11 November 2021 1,251 2 View

Fresh frozen cryostat section immunohistochemistry?

Hi all, I sectioned fresh frozen brains (CO2 euthanized rats) using a cryostat. I typically do immunohistochemistry with perfused free-floating brains, but I want to try it with fresh frozen...

03 August 2016 9,099 13 View

Has anyone traveled with brain sections (already coverslipped) internationally?

Hi all, I have brain sections that have been stained and coverslipped and mounted in the US. I want to scan these brains using a scanner in Germany, and I was wondering how people usually bring...

16 September 2015 1,068 11 View