Andres Diaz

7 Questions 3 Answers 0 Followers

Questions related from Andres Diaz

Unable to detect IL-18 in human serum (Western Blot)?

Hello everyone! I am currently having quite a hard time trying to detect IL-18 in human serum by Western Blot and I honestly do not know what can I improve in my technique or what can I modify so...

10 October 2018 4,886 3 View

Red top tube or SST? Which would work better?

Hello everyone! This is, probably, a common question but I would like to know what do you think about which BD Vaccutainer tube to use: yellow top (with serum separator gel) or red top (with...

03 March 2018 5,039 0 View

Western Blots and X-Ray films...?

Hello, everybody! I am starting a brand new project in which I must image Western Blot membranes developed with a chemiluminescent substrate for HRP. I have previously done this procedure,...

01 January 2018 1,537 1 View

Economic antibodies, worth it?

Hello, everyone. I am currently starting a new project and I would like to know your opinion about the products of a company named "antibodies-online[dot]com" as they seem to offer very affordable...

06 June 2017 2,507 3 View

Why would my recombinant protein not express?

Hello, everybody. I have been having a serious hard time expressing a recombinant protein and I would like to check any source I can in order to achieve some results in my project. Herein I will...

12 December 2016 5,902 5 View

Hard time in protein expression?

Hello, everyone. I'm currently developing protein expression assays with E. coli BL21 AI strain and the pUC-57 vector system. My protein is under regulation of a T5 promoter (IPTG inducible) and...

09 September 2016 8,376 6 View

Is there any possibility to find a relation between an enzyme's affinity for a substrate and its kM?

I wonder if it is possible to determine a correlation between these properties, Do you have any references in regards? Thanks in advance for your answers.

07 July 2014 6,279 7 View