Anders Lunde

5 Questions 7 Answers 0 Followers

Questions related from Anders Lunde

Whole mouse spinal cord immunolabeling in embryos/neonates. Easy?

Dear fellow scholars, We have a mouse line with eGFP and tdTomato labeled axons going from the brainstem to the spinal cord. We are going to clear the spinal cord (using iDisco) in embryos and...

22 March 2018 470 2 View

What are some reccomended methods to map output targets of (cre defined) neuron population?

Dear fellow scholars, We are working with a specific group of projection neurons from the brainstem to the spinal cord, and we want to somehow label (genetically, immuno etc) its target neurons...

22 March 2018 852 2 View

Does anyone have experience with whole tissue click chemistry?

Dear fellow scholars, We have access to a mouse line in which a specific neuron group is producing "click chemistry" ready proteins (proteins with alkyne group). I want to in a whole brain...

22 March 2018 2,112 4 View

Need a tissue conterstain which is invisible with fluorescence microscopy?

Hello. I am looking for something to stain brain cryosection tissue that is visible in normal light microscopy, but does not yield a significant signal with fluorescence microscopy (in range 405...

25 April 2017 564 3 View

Why do I get unspecific grainy background staining in all my anti-rabbit immunofluorescence in mouse brain tissue?

Dear all, I am doing immunofluorescent staining in mouse embryonic brainstem tissue against many different transcription factors. We use 30 min 4% PFA fixed cryosections of ca 14 um thickness. All...

16 December 2016 3,005 15 View