Anabel Zabala Peñafiel

3 Questions 3 Answers 0 Followers

Questions related from Anabel Zabala Peñafiel

How to use KNIME Software to count and differentiate infected from non-infected macrophages?

I have done Romanowsky stain on macrophages infected with different Leishmania clinical isolates. Now I want to count the infected cells to calculate the endocytic rate (% infected macrophages x...

09 September 2019 7,846 1 View

Can I obtain antigen from L. tarentolae culture and use it for ELISA assays to detect CL and MCL from human blood samples?

I want to standardize an ELISA assay to detect CL (cutaneous leishmaniasis) and MCL (mucocutaneous leishmaniasis) from human blood samples. I have found papers where the antigen is obtained from...

09 September 2015 9,988 1 View

How can I measure or determine the action of a certain compound over the Ca homeostasis on in vitro culture of L. mexicana by flow cytometry?

I have been reading several papers about measurement of certain compounds over calcium homeostasis using Fura 2-AM and Calcium Crimson but all of these are used for fluorescent microscopy. I want...

07 July 2014 7,830 4 View