Amit Kumar

7 Questions 1 Answers 0 Followers

Questions related from Amit Kumar

How to show a protein is being dimerized in SDS-PAGE gel?

A protein dimerizes through CTD. I have expressed CTD of protein with two different tags. Now, I want to show that these CTD of protein dimerize in a pull-down assay. But my doubt is that if the...

30 January 2021 7,712 4 View

Why doesn't yrast colonies come on LT- plate even after performing right procedure?

In Y2H system, I am detecting interaction between two proteins. So co-transformation with plasmids colonies doesn't appear on Leuicine tryptophan lacking plate?

26 July 2018 5,914 2 View

Why Linear DNA is used to transform Pichia pastoris but not circular plasmid ?

I am not able to get the point Why do we use linear DNA after digestion of plasmid ? Why can't we use circular DNA to transform Pichia cells ?

02 November 2017 9,025 5 View

Why do we set up primary and secondary culture of pichia pastoris to make electro competent cells?

I am unable to get the point or principle behind secondary culture from primary culture of Pichia pastoris KM71H ?

24 October 2017 2,766 1 View

How to spread evenly all the vero and C6/36 cell line in culture flask?

I have difficulty every time whenever I subculture C6/36 Cell line and vero cell line. Can anyone suggest how to spread cells evenly in growth media.

29 August 2017 8,624 2 View

How to identify contamination in cell culture flask under microscope for vero cell line ?

I am working with vero cell lines. I am subculturing them to maintain my cell line for my dengue virus infection. But I suspect that there is some contamination as small dots on and some elongated...

17 August 2017 8,995 1 View

How much time it takes to polymerize 1% agarose gel?

I am making 1% agarose gel in TAE buffer. So how much time takes to polymerize so that I can run plasmid DNA perfectly?

31 July 2017 1,501 12 View