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Questions related from Amit Kumar
A protein dimerizes through CTD. I have expressed CTD of protein with two different tags. Now, I want to show that these CTD of protein dimerize in a pull-down assay. But my doubt is that if the...
30 January 2021 7,813 4 View
In Y2H system, I am detecting interaction between two proteins. So co-transformation with plasmids colonies doesn't appear on Leuicine tryptophan lacking plate?
26 July 2018 5,983 2 View
I am not able to get the point Why do we use linear DNA after digestion of plasmid ? Why can't we use circular DNA to transform Pichia cells ?
02 November 2017 9,062 5 View
I am unable to get the point or principle behind secondary culture from primary culture of Pichia pastoris KM71H ?
24 October 2017 2,806 1 View
I have difficulty every time whenever I subculture C6/36 Cell line and vero cell line. Can anyone suggest how to spread cells evenly in growth media.
29 August 2017 8,679 2 View
I am working with vero cell lines. I am subculturing them to maintain my cell line for my dengue virus infection. But I suspect that there is some contamination as small dots on and some elongated...
17 August 2017 9,028 1 View
When we isolate plasmid from bacterial culture, usually lb medium is discarded after we pellet down bacterial cells. So my query is what if I directly add TEG buffer to LB medium containing...
08 August 2017 3,950 2 View
I am making 1% agarose gel in TAE buffer. So how much time takes to polymerize so that I can run plasmid DNA perfectly?
31 July 2017 1,560 12 View
