Amirhossein Mohajeri Khorasani

4 Questions 5 Answers 0 Followers

Questions related from Amirhossein Mohajeri Khorasani

Real-Time PCR Troubleshooting: Amplicon Identity, Curve Overlap, and Melting Temperature Discrepancies?

I am encountering several issues in my real-time PCR experiments and seeking guidance on troubleshooting: 1. Inconsistent Amplification Curve Overlap: The amplification curves in my real-time...

09 October 2023 4,914 0 View

Variability in cDNA Synthesis and PCR Results: Impact on Real-Time PCR?

I recently conducted cDNA synthesis followed by conventional PCR for quality assessment. In the course of this experiment, I observed some unexpected results that have raised questions about the...

10 September 2023 993 5 View

Smear and Absence of 28s and 18s Bands in Total RNA Electrophoresis, What Can I do?

During the electrophoresis run on a 1% agarose gel at 100 volts for 25 minutes, I was unable to clearly visualize the expected 28S and 18S bands representing ribosomal RNA. Instead, a smear-like...

28 June 2023 510 5 View

What could be the cause of the high A260/A280 ratio (2.48) and the very low-strength 28s and 18s bands observed in the agarose 1% gel electrophoresis?

I am currently facing a challenge related to the extraction of total RNA from 40 mg colon tissue, and I would greatly appreciate your expertise and insights in troubleshooting the issue. During...

20 June 2023 9,501 3 View