Amir Mohammed Alsharabasy

5 Questions 18 Answers 0 Followers

Questions related from Amir Mohammed Alsharabasy

A problem with seeding fibroblasts in collagen hydrogel?

Previously, I used to seed primary dermal fibroblasts (which initially had slow growth rate) in collagen hydrogel and the cells used to show complete attachment and the normal shape of fibroblasts...

14 October 2018 3,835 4 View

Slow degradation of Sodium Nitroprusside. Any suggestions?

I want to perform an experiment on sodium nitroprusside in vitro, and need to decelerate its degradation. Are there any suggestions for how to achieve that?

12 September 2018 3,469 0 View

How can we concentrate a partitioned protein solution?

Using PEG/salt aqueous two phase extraction system, after the partitioning of protein to either the polymer or salt phase, which are the suitable methods for concentrating of these proteins? Any...

09 October 2017 9,040 14 View

Can we use Trypsin instead of Pepsin in the digestion of the extracellular Matrix towards hydrogel preparation?

In all protocols for the synthesis of ECM hydrogel, the matrix is digested at first with pepsin. Currently, we can't get pepsin for such procedure. Can trypsin enzyme, used in cell culture, be...

24 July 2017 5,791 7 View

What is the percentage of Boron in the bovine bone?

During the EDX analysis of normal and demineralized bovine bone powder, the Ca & P element percentages were as expected in the normal bone and decreased with demineralization. The measured...

26 November 2016 1,470 5 View