11 Questions 46 Answers 0 Followers
Questions related from Amir Mohammed Alsharabasy
I want to model the interaction between different oxidation states of iron and a ligand for optimization and calculation of energy, and wonder how I can define these states with Spartan,...
14 December 2018 3,708 4 View
Previously, I used to seed primary dermal fibroblasts (which initially had slow growth rate) in collagen hydrogel and the cells used to show complete attachment and the normal shape of fibroblasts...
14 October 2018 3,903 4 View
I want to perform an experiment on sodium nitroprusside in vitro, and need to decelerate its degradation. Are there any suggestions for how to achieve that?
12 September 2018 3,526 0 View
I am performing a sequence of modeling steps using Spartan which takes a long time, and wonder if I can pause the calculations, and run again from the end point.
05 September 2018 8,562 2 View
Countless number of papers have discussed the importance of NO with certain low levels and how they modulate the cell proliferation as well as the migration of tumor cells. Other papers have...
09 February 2018 5,042 5 View
I want to make an ether bond between the benzene ring of phenol and a hydrocarbon chain containing terminal double bond for further biomedical applications. How can I activate this bonding?
01 February 2018 1,699 2 View
I am testing the protein concentration using Bradford assay where the absorption of the product should be measured at 590-595 nm. My spectrometer can't reach this range, but the maximum wavelength...
05 November 2017 3,079 1 View
Using PEG/salt aqueous two phase extraction system, after the partitioning of protein to either the polymer or salt phase, which are the suitable methods for concentrating of these proteins? Any...
09 October 2017 9,123 14 View
In all protocols for the synthesis of ECM hydrogel, the matrix is digested at first with pepsin. Currently, we can't get pepsin for such procedure. Can trypsin enzyme, used in cell culture, be...
24 July 2017 5,875 7 View
I want to detect the chemical composition, especially the elemental composition of petroleum jelly for further biomedical applications. How can I prepare the sample for testing using...
23 July 2017 160 5 View
During the EDX analysis of normal and demineralized bovine bone powder, the Ca & P element percentages were as expected in the normal bone and decreased with demineralization. The measured...
26 November 2016 1,532 5 View
