Amel Kevin Alame-Emane

4 Questions 3 Answers 0 Followers

Questions related from Amel Kevin Alame-Emane

What is the best software to perform Whole Genome Sequencing?

Hi ! In my lab, we plan to do Whole Genome Sequencing (WGS), can someone tell me the best dedicated software for this work? I thought I understood that these software require having super...

24 May 2017 5,603 3 View

Spoligotyping: how can I determine that a sapcer is defective?

I ordered twice some differents spacer and I made three diiferents spoligotyping membrane, but these spacer do not hang on the membrane. Any idea ? I suspect that the 5' amino-linker is absent....

21 May 2015 565 4 View

In the case of presence of unspecifics bands in a PCR product, can a dilution improve the quality of sequencing ?

I maked some PCR of rpoB gene (Mycobacterium tuberculosis). I obtained my interest fragment of 411 bp together with some unspecifics bands that disturb sequencing step: the qualities of...

16 March 2015 5,691 4 View

What is the appropriate way to design primers with specific SNPs?

I want to design some primers to characterize differents SNP (Single Nucleotid Polymorphisme) in a simple process using PCR and agarose gel, not for sequencing. What could be the appropriate way...

01 January 1970 9,894 6 View