Alyssa Kim

4 Questions 4 Answers 0 Followers

Questions related from Alyssa Kim

What happens if you accidentally stopped the western blot run for about one hour and then restart?

I am new to western blot and when I was running it today, the machine stopped the run by itself and I realized it only one hour after. The ladder was barely separated, so I restarted the run until...

18 January 2021 5,650 1 View

For MTT assays, are biological replicates just multiple plates of the same set up and condition?

I am new to MTT assays and research in general. I am performing a MTT assay of the same cell line with 4 different conditions. How would you do biological replicates in this situation? I know I...

31 December 2020 1,848 3 View

Is my math correct for protein reconstitution for western blot?

Hi! I'm brand new to western blots and still learning how to calculate the concentration and how to reconstitute lyophilized proteins. I have a 20ug vial I want to reconstitute, and we normally...

31 December 2020 9,911 3 View

How to determine drug concentration for protein inhibition?

Hi! I am doing an experiment where I inhibit a specific protein with a drug and then measuring cell viability. This drug has been used in literature in similar studies. However, I am not sure how...

15 December 2020 4,391 2 View