Allen Eastlund

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Questions related from Allen Eastlund

Looking for resources on multimeric enzyme kinetics

My group works on the pentameric ring motor of the bacteriophage phi29. We are trying to get some bulk assays off the ground to study it's in vitro enery use (MESG-based ATPase assays) but while...

07 July 2017 871 2 View

T4 genome extraction using phenol:chloroform forms bundle after rehydration

We are trying to extract genomic DNA from T4 but the end product after attempting resuspension in milli-water is a cloudy 'bundle' of DNA that will not dissolve into solution. General process:...

02 February 2017 8,392 3 View

Are there fluorimetric ATPase assays available?

I currently use an absorbance-based ATPase assay centered around coupling released phosphates with the conversion of MESG by PNP. This results in increased product concentrations and a...

12 December 2015 888 3 View

Can samples sublimate in longer term -80 storage?

I have a protein stock that is stored in 1.5 mL eppendorf tubes that I originally (approx 1 year ago) aliquoted at 300mL/tube. I'm curious if kept in the closed tubes for long term storage, can...

06 June 2013 4,122 26 View