Alice Gentil Dit Maurin

7 Questions 11 Answers 0 Followers

Questions related from Alice Gentil Dit Maurin

Amino Acid distance into crystallized protein?

Hello, Is someone know if it's possible from a crystallized protein modelisation to know the distance between X and Y amino acid? Which softwares/ websites to consider? Thanks a lot in...

06 June 2019 8,343 4 View

Detection of PAK proteins in HL60 cell line using western-blot?

Hello, I am trying to detect PAK1, PAK2 in cell lysates from HL60 cell line in western-blot. I tried to go for 50ug of protein but I just had a very tiny little band. I thought 50 ug of prot...

07 July 2018 9,294 0 View

NIH3T3 in Soft agar assay, max time of incubation?

Hi all, I am using the CellBioLabs kit to do my soft agar assay. I am quite surprised to see in the protocol the time incubation for NIH3T3-Ras transfected cells is 8 days? It seems to me quite...

07 July 2016 4,831 2 View

Add Complete med. above my cell agar layer?

Hi all, I was wondering why my positive control (NIH/3T3 + RasG12V transfection) doesn't form colonies on my soft agar assay... Just after the gelation of the second layer (cell/medium/agar, 15...

07 July 2016 9,713 1 View

Ras G12V mutant issue in Soft agar assay?

Hi All, I am starting to do some soft agar assays using NIH/3T3 cells. As positive control I used NIH transfected with RasG12V plasmid. Before placing my cell on the agar gel I noticed (twice and...

07 July 2016 9,811 1 View

Does anyone has expereinces in NIH 3T3 electroporation using NEON system fron Invitrogen?

Hi everyone! I did a first experience in NIH 3T3 using Invitrogene Neon system: 1x10.6 cell/transfection, eletropo program ; Pulse voltage 1.350V, pulse width ms 20, pulse number 2 (100ul tip)....

06 June 2016 7,429 2 View

Jurkat activation by LPS?

Hi, I was wondering if we can activate Jurkat cells with LPS (normally yes as they would have TLR4). What molecule we could analyse to monitor the activation? TNFa? Others? By ELISA (Which...

06 June 2016 7,903 2 View