Alexia Hyde

4 Questions 4 Answers 0 Followers

Questions related from Alexia Hyde

Can two genes have the same melt curve profile (for RT-qPCR)?

I am testing out 4 different genes (coding for completely different proteins). When I ran an RT-qPCR assay with these genes (all on the same plate, on the same day), I noticed that a couple of the...

31 August 2021 9,690 4 View

CDNA prep stable on ice?

I was going to put my cDNA prep in the thermocycler when I noticed someone swooped in and started using it before me. I don't have access to another thermocycler and this other person's program...

11 November 2019 5,366 2 View

Can blood plasma be stored (in a -80 freezer) in regular microcentrifuge tubes or should I keep it in an EDTA-coated tube?

I will be collecting blood samples from rats (in EDTA-coated tubes) and centrifuging to extract the plasma. My question is: what type of tube should I be using to aliquot the plasma for long-term...

08 April 2019 4,964 3 View

IL-1beta ELISA with brain tissue homogenates - Why am I not getting any signal in my samples (but my standard curve looks good)?

I am trying to detect IL-1 beta in brain tissue homogenates (rat hippocampus and striatum) using an ELISA kit (R&D) but I am not getting any signal in my samples, even though I am able to get...

01 January 2018 6,437 5 View