Alexandra Walsh

6 Questions 6 Answers 0 Followers

Questions related from Alexandra Walsh

Removing magnesium cofactor from enzyme?

I am working with vanadium dependent bromoperoxidase which has vanadium in its active site and (presumingly) several magnesium ions attached which do not take part in the catalytic reactions of...

02 February 2017 9,585 3 View

How can I seed cell culture on CaF2 windows?

I need a CaF2 surface for a cell culture that will subsequently be examined by confocal Raman spectroscopy. Currently I am working with something like this...

05 May 2015 4,816 3 View

How long do mononuclear phagocytic cells survive after treatment with different doxorubicin formulations?

I have recently learned that mononuclear phagocytic cells (monocytes) readily engulf liposomal doxorubicin, both pegylated and non-pegylated, albeit the phagocytic activity is less towards...

08 August 2014 4,690 2 View

Which molarity should my borate buffer have?

I recently learned that in order to improve the cell adhesive properties of Poly-D-lysine (PDL) one could try to solve the powder in a alkaline buffer instead of dH2O/MQH2O. Which molarity should...

10 October 2013 2,964 1 View

Mycoplasma pneumonia infection?

I have read that most common humane mycoplasma species that infect cell cultures are M. fermentans and M. orale. Does anyone of you have encountered infections by M. pneumonia? If yes, what could...

09 September 2013 9,272 4 View

Is it possible to coat intracelluar gold nano-colloids?

I am working with Raman imaging of living cells and recently read that you could grow gold nanoparticles intracellularly for improved SERS enhancements and decrease of outflux of the nanoparticles...

08 August 2013 9,752 5 View