Alex Salam

4 Questions 7 Answers 0 Followers

Questions related from Alex Salam

How to elute virus from dried blood spots for purpose of viral culture?

Hi. I am collected blood from patients with a BSL-4 virus onto filter paper, i.e dried blood spots. We are running some validation experiments to see whether the drying process inactive the virus....

06 August 2019 780 3 View

Why is red cell lysing solution lysing my PBMCs?

I am isolating PBMCs with the use of cell protection tubes. After a couple of washes, I get a nice cell pellet. I then add red cell lysing solution to the pellet, but this seems to be lysing my...

30 January 2019 4,449 2 View

Is it possible to use Qiagen RNA extraction kits on cells that have been frozen in Trizol for RNA preservation?

I am storing PBMCs for a long time before analysing gene expression. These PBMCs will have a BSL category 4 virus within them. So I need to inactivate the virus before processing the samples....

06 September 2018 5,195 2 View

Can you freeze whole blood, and then subsequently isolate PBMCs?

Hi, we are working in a very resource limited setting where the lab resources on site are very basic. We are doing serial blood sampling for drug concentrations, which includes overnight samples....

09 May 2018 993 11 View