Alessio Balzerano

2 Questions 1 Answers 0 Followers

Questions related from Alessio Balzerano

Does anybody know a mammalian membrane protein extraction protocol?

Hi everybody, I need to extract internal-associated membrane proteins from mammalian cell lines, eventually without using ultracentrifuge. My aim is to analyze protein expression by...

09 September 2017 1,620 4 View

Which recombinant protein is better to use for in vitro Ubiquitination assay?

Hi everybody, I need to perform an in vitro ubiquitination assay, using a recombinant protein as substrate. I would like to know if is better a protein produced in Sf9 Baculovirus cells or in...

09 September 2017 7,019 1 View