Alejandro Escobar

3 Questions 3 Answers 0 Followers

Questions related from Alejandro Escobar

Does anyone know a protocol for expand or generate CD4+ T cells against TT, PPD or Flu from PBMC?

Hi, I need a protocol to this aim, many years ago I saw a very easy protocol to expand lymphocytes from PBMC using a Flu peptide, but now I have not access to this protocol. If someone has...

06 June 2017 10,090 5 View

How do I measure intracellular IL-1?

IL-1 has not a typical secretory pathway, so If I want to detect IL-1 intracellular, can I use the classical protocols to assess it (I mean golgi stop)? regards

01 January 2016 259 2 View

Does anyone know about variant polarized M3 macrophages with primary immunoregulatory roles as a result of IL-10 production?

In response to various signals, MΦ may undergo classical M1 activation or alternative M2 activation. The M1 phenotype is characterized by the expression of high levels of proinflammatory...

06 June 2014 1,488 1 View